Resumo A covid-19 resultou na implementação de medidas de distanciamento social e fechamento de escolas no mundo. Este estudo explora alguns dos impactos, tais como escolhas pedagógicas, reflexões e barreiras do professor quando ele repentinamente mudou para um ambiente de ensino online e teve que ministrar aulas de Educação Física (EF) com o uso da tecnologia (Google Sites). A metodologia do autoestudo com análise temática foi utilizada para investigar as experiências do autor principal. Achados destacam como o professor teve que encontrar novas formas de ensino online, fazendo com que os alunos não estivessem exclusivamente em frente à tela, e atendesse as características sociais e dinâmicas da EF. Finalmente, as experiências e o processo de reflexão demonstraram a necessidade de ser coerente com as escolhas pedagógicas e abordagens teóricas e práticas como professor. Este artigo contribui para futuras formas de ensino de EF online, ao compreender a práxis de um professor.

Abstract Covid-19 has resulted in the implementation of social distancing measures and school closures worldwide. This study explores some of the impacts, such as pedagogies, teaching strategies, reflections, and barriers of the teacher as he suddenly moved to an online teaching environment and taught PE lessons with the use of technology (Google Sites). A self-study methodology with thematic analysis was used to investigate the lead author’s experiences. Findings and discussions highlight how the teacher had to find new forms of teaching online, ways of making the students not be exclusively in front of the screen, and simultaneously be concordant with the social and dynamic characteristics of PE. Ultimately, the experiences and the process of reflection demonstrated the need to be coherent with teaching beliefs, assumptions, theoretical approaches and practices as a teacher. This article foregrounds and contributes to future ways of teaching PE online by understanding one teacher’s praxis.

Resumen El Covid-19 ha llevado a la implementación de medidas de distancia social y al cierre de escuelas en todo el mundo. Este estudio explora algunos de los impactos, como opciones pedagógicas, reflexiones y barreras del profesor cuando este cambia repentinamente a un entorno de enseñanza online, con el uso de la tecnología (Google Sites). La metodología de self-study con análisis temático se utilizó para investigar las experiencias del autor principal. Los resultados destacan que el profesor tuvo que encontrar nuevas formas de enseñanza online, logrando que los alumnos no estuvieran exclusivamente frente a la pantalla, y cumplir con las características sociales y dinámicas de la Educación Física. Finalmente, las experiencias y la reflexión demostraron la necesidad de ser coherente, como profesor, con las elecciones pedagógicas y los enfoques teóricos y prácticos. Este artículo contribuye con las futuras formas de enseñanza de la Educación Física online mediante la comprensión de la praxis de un profesor.

Objective to analyze the available evidence on the main contributions of the birth plan as an instrument of good obstetric practices in the process of delivery and birth in a hospital environment. Method an integrative review with 12 articles published between 2013 and 2018 in the MEDLINE, Virtual Health Library, LILACS, SciELO and PubMed databases, using full and complete original works, reflection, update and experience report. Results the importance of the birth plan importance was observed to encourage good obstetric practices, allow greater autonomy and freedom of women’s choice, contribute to a qualified and humanized care, facilitate women's trust with the team, and promote greater satisfaction with childbirth, communication with the involved professionals and provide more satisfactory maternal/neonatal results. Conclusion it was proved that the birth plan is an instrument that provides good obstetric practices in the process of delivery and birth in a hospital environment.

Objetivo analizar las evidencias disponibles sobre las principales contribuciones del plan de parto como instrumento de las buenas prácticas obstétricas en el proceso de parto y nacimiento en el entorno de un hospital. Método revisión integradora con 12 artículos publicados entre 2013 y 2018 en las siguientes bases de datos: MEDLINE, Biblioteca Virtual de Saúde, LILACS, SciELO y PubMed, utilizando trabajos originales, de reflexión, de actualización y reporte de experiencias, completos y disponibles en forma íntegra. Resultados se observó la importancia del plan de parto para estimular las buenas prácticas obstétricas, permitir mayor autonomía y libertad de elección de las mujeres, contribuir a una asistencia calificada y humanizada, facilitar que la mujer confíe en el equipo médico, además de favorecer una mayor satisfacción con el parto, la comunicación con los profesionales participantes, y propiciar resultados maternos/neonatales más satisfactorios. Conclusión se comprobó que el plan de parto es un instrumento que propicia buenas prácticas obstétricas en el proceso de parto y nacimiento en un ambiente de hospital.

Objetivo analisar as evidências disponíveis sobre as principais contribuições do plano de parto como instrumento das boas práticas obstétricas no processo de parto e nascimento em ambiente hospitalar. Método revisão integrativa com 12 artigos publicados entre 2013 e 2018 nas bases de dados MEDLINE, Biblioteca Virtual de Saúde, LILACS, SciELO e PubMed, utilizando trabalhos originais, reflexão, atualização e relato de experiência, completos e disponíveis na íntegra. Resultados observou-se a importância do plano de parto para estimular as boas práticas obstétricas, permitir maior autonomia e liberdade de escolha das mulheres, contribuir para um atendimento qualificado e humanizado, facilitar a confiança da mulher com a equipe, além de favorecer maior satisfação com o parto, comunicação com os profissionais envolvidos e propiciar resultados maternos/neonatais mais satisfatórios. Conclusão comprovou-se que o plano de parto é um instrumento que propicia boas práticas obstétricas no processo de parto e nascimento em ambiente hospitalar.

Objetivou-se com esse estudo detectar o DNA genômico de T. gondii em amostras de testículo e epidídimo de ovinos comercializados em abatedouros do Estado de Pernambuco Região Nordeste do Brasil. Foram coletadas 50 amostras de soro sanguíneo, 50 amostras de testículos e 50 de epidídimos. Para a triagem dos animais foi utilizada a técnica de Imunofluorescência Indireta (RIFI) e posteriormente empregou-se a Reação em Cadeia da Polimerase (PCR) nos animais positivos na sorologia. Observou-se 24% (12/50) dos animais positivos na RIFI e o DNA genômico foi detectado no epidídimo em 8,3% (1/12) das amostras. A identidade molecular dos produtos amplificados foi confirmada por sequenciamento. Relata-se a primeira ocorrência da presença do DNA de T. gondii em órgãos do sistema reprodutivo de carneiros naturalmente infectados no Brasil.

The aim of the study was to detect genomic DNA of Toxoplasma gondii in testicle and epididymis samples from rams sold in abattoirs in the state of Pernambuco, Northeast Brazil. Fifty (50) blood serum samples were collected, as well as 50 testicle and epididymis samples. Indirect Immunofluorescence (IIF) was used during screening of the rams. The Polymerase Chain Reaction (PCR) was used with animals that were positive in serology. Our results confirmed that 24% (12/50) of the rams were positive in IIF. Genomic DNA was detected in the epididymis at 8.3% (1/12) of the animals. The molecular identity of the amplified products was confirmed through sequencing. This paper reports the first occurrence of T. gondii DNA in the reproductive organs of naturally infected rams in Brazil.

This report describes the development of a SYBR Green I based real time polymerase chain reaction (PCR) protocol for detection on the ABI Prism 7000 instrument. Primers targeting the gene encoding the SSU rRNA were designed to amplify with high specificity DNA from Schistosoma mansoni, in a real time quantitative PCR system. The limit of detection of parasite DNA for the system was 10 fg of purified genomic DNA, that means less than the equivalent to one parasite cell (genome ~580 fg DNA). The efficiency was 0.99 and the correlation coefficient (R²) was 0.97. When different copy numbers of the target amplicon were used as standards, the assay could detect at least 10 copies of the specific target. The primers used were designed to amplify a 106 bp DNA fragment (Tm 83ºC). The assay was highly specific for S. mansoni, and did not recognize DNA from closely related non-schistosome trematodes. The real time PCR allowed for accurate quantification of S. mansoni DNA and no time-consuming post-PCR detection of amplification products by gel electrophoresis was required. The assay is potentially able to quantify S. mansoni DNA (and indirectly parasite burden) in a number of samples, such as snail tissue, serum and feces from patients, and cercaria infested water. Thus, these PCR protocols have potential to be used as tools for monitoring of schistosome transmission and quantitative diagnosis of human infection.

The detection of specific DNA sequences by polymerase chain reaction (PCR) has proved extremely valuable for the analysis of genetic disorders and the diagnosis of a variety of infectious disease pathogens. However, the application to the detection of Schistosoma mansoni is rare, despite a recommendation of the World Health Organization that a major focus of research on schistosomiasis should be on the development and evaluation of new strategies and tools for control of the disease. In this context, a few studies were published for the detection of the parasite in snails, monitoring of cercariae in water bodies, and diagnosis of human infection. The present minireview describes sensitive and specific PCR based systems to detect S. mansoni, indicating possible applications in the detection of snail infection, monitoring of transmission sites, and diagnosis of human infection.